Universal Stem-Loop Primer Method for Screening and Quantification of MicroRNA
نویسندگان
چکیده
منابع مشابه
Universal Stem-Loop Primer Method for Screening and Quantification of MicroRNA
RT-qPCR is the accepted technique for the quantification of microRNA (miR) expression: however, stem-loop RT-PCR, the most frequently used method for quantification of miRs, is time- and reagent-consuming as well as inconvenient for scanning. We established a new method called 'universal stem-loop primer' (USLP) with 8 random nucleotides instead of a specific sequence at the 3' end of the tradi...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2014
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0115293